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Image Search Results
Journal: Materials Today Bio
Article Title: Uniform and controllable surface nano-structure on polyetheretherketone implants can regulate mechanical property to enhance soft tissue integration through Piezo1/TGF-β1 signaling axis
doi: 10.1016/j.mtbio.2025.101645
Figure Lengend Snippet: The impact of nanoscale modification of PEEK implant surfaces on the mechanical perception, proliferation, and expression of fibrosis-related proteins in L929 cells. (A) The expression levels of mechanical perception, proliferation, and fibrosis-related proteins were evaluated in L929 cells cultured on different nanoscale modified PEEK surfaces at multiple time points. (B) The secretion of TGF-β1 from L929 cells cultured on various nanoparticle-modified PEEK surfaces was quantified using Elisa at multiple time points. (C) Immunofluorescence analysis was conducted to assess the expression differences of Piezo1, ITGB1, and α-SMA in L929 cells cultured on different nanoparticle-modified PEEK surfaces. (D) Quantitative fluorescence analysis was performed on the data presented in C.
Article Snippet:
Techniques: Modification, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Fluorescence
Journal: Materials Today Bio
Article Title: Uniform and controllable surface nano-structure on polyetheretherketone implants can regulate mechanical property to enhance soft tissue integration through Piezo1/TGF-β1 signaling axis
doi: 10.1016/j.mtbio.2025.101645
Figure Lengend Snippet: Piezo1 mediates the proliferation and fibrosis enhancement of L929 cells on PEEK implants modified with a 500 nm surface treatment. (A) Changes in calcium influx induced by the specific Piezo1 agonist (Yoda1) and the calcium channel inhibitor (GsMTx-4) were assessed using Fluo-4AM. (B) The fluorescence intensity of calcium accumulation in L929 cells following treatment with Yoda1 and GsMTx-4 was observed under a fluorescence microscope. (C) Quantitative analysis of the average fluorescence intensity presented in B. (D) The regulatory effects of Piezo1 on proliferation-related pathways, fibrosis processes, and adhesion-associated proteins were evaluated separately on PEEK and PEEK-500. (E) RT-qPCR was employed to assess the transcriptional differences in fibrosis-related genes in L929 cells following the activation or inhibition of Piezo1. (F) Changes in TGF-β1 secretion by L929 cells after the activation or inhibition of Piezo1 were analyzed. (G) The involvement of TGF-β1 in fibrosis regulation through the activation and upregulation of Piezo1 was confirmed. (H) Schematic representation of the mechanism by which L929 cells detect mechanical signals from PEEK samples to facilitate soft tissue integration.
Article Snippet:
Techniques: Modification, Fluorescence, Microscopy, Quantitative RT-PCR, Activation Assay, Inhibition